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Glycan Analysis
Product Listing
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Description:
STREAMLINED, RAPID and RELIABLE HPLC and LC-MS-Ready SAMPLE PREPARATION of O-GLYCANS EZGlyco® O-Glycan Prep Kit provides a streamlined, rapid and reliable O-glycan sample preparation. The complete kit includes all reagents to release, enrich, label and purify O-glycans for HPLC and LC-MS analysis. The kit adopts a unique O-glycan releasing method “Eliminative Oximation” using a high reactive amine and an organic superbase, which yields high recovery and less degradation (peeling) of released O-glycans. Applications include characterization of O-glycosylation of recombinant glycoproteins in upstream R&D, quality control and process control. It is also suited for O-glycan biomarker discovery in biological samples such as mucins, serum and exosome, and O-glycan analysis of functional foods such as dairy products. Content: Protocol Glycan Release Reagent A Glycan Release Reagent B Glycan Capturing Beads Filter Column 2-Aminobezamide Reducing Reagent Cleanup Column O-GLYCAN SAMPLE PREP IN 5 HOURS O-Glycan sample preparation is completed in 5 hours with its unique O-glycan releasing reagents. Thus, HPLC/LC-MS analysis is achieved on the same day. HIGH RECOVERY A combination of the unique O-glycan releasing reagent and the new O-Glycan Capturing Beads enables high recovery of released O-glycans from various glycoproteins. MINIMIZE “PEELING” Glycan releasing reagents minimize decomposition of O-glycans (peeling), resulting in O-glycan characterization with higher accuracy. EASY AND SAFE OPERATION With a simple protocol to follow, O-glycan sample preparation is completed without any special laboratory equipment and toxic reagents.
OUT OF STOCKSTREAMLINED, RAPID and RELIABLE HPLC and LC-MS-Ready SAMPLE PREPARATION of O-GLYCANS EZGlyco® O-Glycan Prep Kit provides a streamlined, rapid and reliable O-glycan sample preparation. The complete kit includes all reagents to release, enrich, label and purify O-glycans for HPLC and LC-MS analysis. The kit adopts a unique O-glycan releasing method “Eliminative Oximation” using a high reactive amine and an organic superbase, which yields high recovery and less degradation (peeling) of released O-glycans. Applications include characterization of O-glycosylation of recombinant glycoproteins in upstream R&D, quality control and process control. It is also suited for O-glycan biomarker discovery in biological samples such as mucins, serum and exosome, and O-glycan analysis of functional foods such as dairy products. Content: Protocol Glycan Release Reagent A Glycan Release Reagent B Glycan Capturing Beads Filter Column 2-Aminobezamide Reducing Reagent Cleanup Column O-GLYCAN SAMPLE PREP IN 5 HOURS O-Glycan sample preparation is completed in 5 hours with its unique O-glycan releasing reagents. Thus, HPLC/LC-MS analysis is achieved on the same day. HIGH RECOVERY A combination of the unique O-glycan releasing reagent and the new O-Glycan Capturing Beads enables high recovery of released O-glycans from various glycoproteins. MINIMIZE “PEELING” Glycan releasing reagents minimize decomposition of O-glycans (peeling), resulting in O-glycan characterization with higher accuracy. EASY AND SAFE OPERATION With a simple protocol to follow, O-glycan sample preparation is completed without any special laboratory equipment and toxic reagents.
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Description:
BlotGlyco® Beads: Polymer beads are densely covered with hydrazide groups to facilitate glycan capture BlotGlyco® is a sample preparation kit for glycan analysis of biological samples. Using this kit will purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on. BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities. BlotGlyco® kit is a useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.
OUT OF STOCKBlotGlyco® Beads: Polymer beads are densely covered with hydrazide groups to facilitate glycan capture BlotGlyco® is a sample preparation kit for glycan analysis of biological samples. Using this kit will purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on. BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities. BlotGlyco® kit is a useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.
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Description:
BlotGlyco® Beads: Polymer beads are densely covered with hydrazide groups to facilitate glycan capture BlotGlyco® is a sample preparation kit for glycan analysis of biological samples. Using this kit will purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on. BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities. BlotGlyco® kit is a useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.
OUT OF STOCKBlotGlyco® Beads: Polymer beads are densely covered with hydrazide groups to facilitate glycan capture BlotGlyco® is a sample preparation kit for glycan analysis of biological samples. Using this kit will purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on. BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities. BlotGlyco® kit is a useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.
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Description:
BlotGlyco® Beads: Polymer beads are densely covered with hydrazide groups to facilitate glycan capture BlotGlyco® is a sample preparation kit for glycan analysis of biological samples. Using this kit will purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on. BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities. BlotGlyco® kit is a useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.
OUT OF STOCKBlotGlyco® Beads: Polymer beads are densely covered with hydrazide groups to facilitate glycan capture BlotGlyco® is a sample preparation kit for glycan analysis of biological samples. Using this kit will purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on. BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities. BlotGlyco® kit is a useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.
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Description:
BlotGlyco® Beads: Polymer beads are densely covered with hydrazide groups to facilitate glycan capture BlotGlyco® is a sample preparation kit for glycan analysis of biological samples. Using this kit will purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on. BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities. BlotGlyco® kit is a useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.
OUT OF STOCKBlotGlyco® Beads: Polymer beads are densely covered with hydrazide groups to facilitate glycan capture BlotGlyco® is a sample preparation kit for glycan analysis of biological samples. Using this kit will purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on. BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities. BlotGlyco® kit is a useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.
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Description:
BlotGlyco® Beads: Polymer beads are densely covered with hydrazide groups to facilitate glycan capture BlotGlyco® is a sample preparation kit for glycan analysis of biological samples. Using this kit will purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on. BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities. BlotGlyco® kit is a useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.
OUT OF STOCKBlotGlyco® Beads: Polymer beads are densely covered with hydrazide groups to facilitate glycan capture BlotGlyco® is a sample preparation kit for glycan analysis of biological samples. Using this kit will purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on. BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities. BlotGlyco® kit is a useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.
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Description:
BlotGlyco® Beads: Polymer beads are densely covered with hydrazide groups to facilitate glycan capture BlotGlyco® is a sample preparation kit for glycan analysis of biological samples. Using this kit will purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on. BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities. BlotGlyco® kit is a useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.
OUT OF STOCKBlotGlyco® Beads: Polymer beads are densely covered with hydrazide groups to facilitate glycan capture BlotGlyco® is a sample preparation kit for glycan analysis of biological samples. Using this kit will purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on. BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities. BlotGlyco® kit is a useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.
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Description:
BlotGlyco® Beads: Polymer beads are densely covered with hydrazide groups to facilitate glycan capture BlotGlyco® is a sample preparation kit for glycan analysis of biological samples. Using this kit will purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on. BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities. BlotGlyco® kit is a useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.
OUT OF STOCKBlotGlyco® Beads: Polymer beads are densely covered with hydrazide groups to facilitate glycan capture BlotGlyco® is a sample preparation kit for glycan analysis of biological samples. Using this kit will purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on. BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities. BlotGlyco® kit is a useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.
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Description:
BlotGlyco® Beads: Polymer beads are densely covered with hydrazide groups to facilitate glycan capture BlotGlyco® is a sample preparation kit for glycan analysis of biological samples. Using this kit will purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on. BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities. BlotGlyco® kit is a useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.
OUT OF STOCKBlotGlyco® Beads: Polymer beads are densely covered with hydrazide groups to facilitate glycan capture BlotGlyco® is a sample preparation kit for glycan analysis of biological samples. Using this kit will purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on. BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities. BlotGlyco® kit is a useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.
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Description:
BlotGlyco® Beads: Polymer beads are densely covered with hydrazide groups to facilitate glycan capture BlotGlyco® is a sample preparation kit for glycan analysis of biological samples. Using this kit will purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on. BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities. BlotGlyco® kit is a useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.
OUT OF STOCKBlotGlyco® Beads: Polymer beads are densely covered with hydrazide groups to facilitate glycan capture BlotGlyco® is a sample preparation kit for glycan analysis of biological samples. Using this kit will purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on. BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities. BlotGlyco® kit is a useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.
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Description:
HPLC-Ready N-glycan Preparation The EZGlyco™ mAb-N Kit with 2-AB is a complete kit which includes the enzyme, reagents, and accessories necessary for completion of HPLC-ready N-glycan preparation in less than 2 hours of handling time. It also provides traditional 2-aminobenzamide labeling designed with a non-toxic reductant. The EZGlyco™ mAb-N Kit with 2-AB is a complete kit which includes the enzyme, reagents, and accessories necessary for completion of HPLC-ready N-glycan preparation in less than 2 hours of handling time. It also provides traditional 2-aminobenzamide labeling designed with a non-toxic reductant. Contents Antibody Capturing Column (Qty 10) Silicone Bottom Cap (Qty 10) Antibody Capturing Solution (Qty 1) Washing Buffer (Qty 1) Glycan Release Enhancer (Qty 1) PNGase F Solution (Qty 1) 2-Aminobenzamide (Qty 3) Reducing Reagent (Qty 3) Cleanup Column (Qty 10) Instruction Manual (Qty 1)
OUT OF STOCKHPLC-Ready N-glycan Preparation The EZGlyco™ mAb-N Kit with 2-AB is a complete kit which includes the enzyme, reagents, and accessories necessary for completion of HPLC-ready N-glycan preparation in less than 2 hours of handling time. It also provides traditional 2-aminobenzamide labeling designed with a non-toxic reductant. The EZGlyco™ mAb-N Kit with 2-AB is a complete kit which includes the enzyme, reagents, and accessories necessary for completion of HPLC-ready N-glycan preparation in less than 2 hours of handling time. It also provides traditional 2-aminobenzamide labeling designed with a non-toxic reductant. Contents Antibody Capturing Column (Qty 10) Silicone Bottom Cap (Qty 10) Antibody Capturing Solution (Qty 1) Washing Buffer (Qty 1) Glycan Release Enhancer (Qty 1) PNGase F Solution (Qty 1) 2-Aminobenzamide (Qty 3) Reducing Reagent (Qty 3) Cleanup Column (Qty 10) Instruction Manual (Qty 1)