1. What is SPE
2. Purification mechanism of SPE
3. Troubleshooting
4. Different types of separation modes

    

What is SPE?

Solid-phase extraction (SPE) is a fundamental and versatile sample preparation technique widely employed in the field of analytical chemistry. Its primary purpose is to isolate and concentrate specific target compound(s) from complex mixtures, enabling enhanced sensitivity and accuracy in subsequent analytical methods. SPE plays a crucial role in sample cleanup, pre-concentration, and purification, making it an indispensable tool for a broad range of applications, including environmental monitoring, pharmaceutical analysis, clinical research, forensic investigations, and food safety testing.

 

Purification mechanism of SPE

The purification mechanism of SPE method can be divided into two modes including target compound retention mode and interference retention mode.

In target compound retention mode, the target compound is retain by the solid sorbent while the interference compounds are being washed off. Subsequently, the target compounds is eluted using an appropriate solvent to give a purified target compound, free from interference compound. This retention mode is also commonly used for concentrating target compound from a diluted sample.

In interference retention mode, interference compounds are retained by the solid sorbent while the target compound passes through the solid sorbent unretained and collected in its pure form.

Back to top

 

Steps of SPE method

The universal steps of SPE method are showed as followed:

Back to top

Flow rate control

The following four methods are commonly used for flow rate control. The choice of method depends on the application and the availability of apparatus.

Back to top

Choose Proper Sorbent

• Sorbent Selection by Matrix and Analyte

Taget Compound	Soluble in organic solvent	Soluble in hexane	Normal phase adsorption	Silica, FL-PR
			Normal phase	PSA, NH2, CN
		Soluble in methanol or methanol/water mixture	Normal phase	PSA, NH2, CN
			Reversed phase	HLB, C18, C8, Ph, C2
	Soluble in aqueous solvent	Non -ionizable	Reversed phase	HLB, C18, C8, Ph, C2
		Ionizable	Cationic	MCX, WCX, SCX
			Anionic	MAX, WAX, SAX

It is a standard of SPE phase selection that the SPE phase should be consistent with the property of the analytes. Besides, it is necessary to consider the characteristics of sample matrix, analytes and the solubility of the solvent used. Solid phase extraction sorbent can be divided into reverse phase, normal phase and ion exchange according to chemical properties.

• Retention Mechanisms

SPE Phase Type	Analyte	Sample Matrix Type	Mechanisms
Reverse Phase	Non-polar Compound	Polar	Reverse phase sorbent is used to extract analytes from aqueous samples using a non-polar (hydrophobic) retention mechanism.
Normal Phase	Polar Compound	Non-polar	Normal phase sorbent is used to extract analytes from non-polar (typically solvent) matrices using polar retention mechanisms.
Ion Exchange	Ionizable Compound	Aqueous, Non-polar, etc	Ion exchange sorbent is used to extract ionizable analytes from aqueous samples using an ion exchange retention mechanism.
Mixed Mode	Ionizable Nonpolar Compound	Aqueous, Non-polar, etc	Mixed mode sorbent is used to extract ionizable analytes from aqueous or nonpolar samples using an ion exchange retention mechanism combined with non-polar retention mechanism.

Back to top

Troubleshooting

• Method Optimization to Improve Analyte Recovery

Low analyte recovery can be caused by low retention of analytes or inefficient retention disruption during the elution step.

1. Low retention of analytes

Issue and Possible Cause	Action
Unsuitable SPE sorbent	Select suitable sorbent with improved retention of analytes
Unsuitable loading condition or washing solvent	Adjust loading condition or washing solvent
Unsuitable loading volume	Reduce loading volume or increase sorbent weight
Sorbent overdried	Concern for sorbent overdrying is critical during experiment

 

2. Inefficient retention disruption during elution

Issue and Possible Cause	Action
The retention of the analyte is too much	Select suitable sorbent with proper retention of analytes
Unsuitable elution solvent	Adjust elution solvent
Unsuitable elution volume	Adjust elution volume

 

3. Tips

Issue and Possible Cause	Action
Insufficient extraction	Adjust extraction method
Unstable analyte	Pay attention to pH manipulation and temperature
Analyte bounded with the impurity	Remove impurities before loading
High impurity content	Reduce loading volume or increase sorbent weight

 

• Method Optimization to Improve Reproducibility

Issue and Possible Cause	Action
Personal error	Try simplified methods
Poor impurity removal ability	Change sample preparation method
Column flow rate is too fast	Control flow rate with the help of SPE Vacuum Manifolds
Sorbent overdried	Re-Conditioning

• Method Optimization to Improve Impurity Removal Ability

1. Adjust the pH of the sample if necessary
2. Adjust the fraction of the washing solvent or elution solvent
3. Choose SPE cartridge with proper sorbent and weight
4. Improve SPE operation

 

• Method Optimization to Control Flow Rate

Issue and Possible Cause	Action
Improper partical size of the sorbent	Choose sorbent with proper partical size
Suspended insoluble residual, such as lipids and proteins	Centrifuge, refrigeration or other sample pretreatment methods before loading
High viscosity liquid samples	Dilute the sample by organic solvent
Improper solvent flow	Adjust valves of SPE Vacuum Manifolds for precise flow control

Back to top

Different types of separation modes

Polymeric Cartridge	Normal Phase	Reverse Phase	Ion-Exchange	Carbon Cartridge	Special Cartridge
SHIMSEN Styra HLB SHIMSEN Styra MCX SHIMSEN Styra WCX SHIMSEN Styra MAX SHIMSEN Styra WAX	SHIMSEN Styra SI SHIMSEN Styra FL-PR SHIMSEN Styra CN SHIMSEN Styra AL-N SHIMSEN Styra AL-A SHIMSEN Styra AL-B SHIMSEN Styra NH2 SHIMSEN Styra PSA	SHIMSEN Styra C18 SHIMSEN Styra C18-U SHIMSEN Styra C8 SHIMSEN Styra C2 SHIMSEN Styra Ph	SHIMSEN Styra SCX SHIMSEN Styra SAX	SHIMSEN Styra GCB SHIMSEN Styra GCB/PSA SHIMSEN Styra GCB/NH2 SHIMSEN Styra GAC SHIMSEN Styra SAX/PSA	SHIMSEN Styra Glass SHIMSEN Styra Na2SO4

Features of Silica-Based Reversed Phase Cartridges

1. The functional groups bonded on the surface of silica gel play a major role in the retention of the target compounds. Based on the types of bonded functional groups, there are three mechanisms by which sorbents retain the target compounds: reversed phase retention, normal phase retention and ion exchange retention.
2. Bonded silica sorbents are stable from pH 2 to 7.5.
3. The bonded silica sorbent are rigid, neither shrink nor expand during solvent conversion, and can reach equilibrium rapidly in new solvents.
4. The matrix of bonded silica sorbents is spherical silica gel with a particle size of 50 μm. The particle size is uniform and its surface is smooth, which reduces the resisting force of the passing solvent. The characteristic pore size of bonded silica sorbents is 60 Å, which are suitable for compounds with molecular weight less than 15,000.
5. Reverse phase sorbents are endcapped, while normal phase sorbents and ion exchange sorbents are not endcapped.

 

SHIMSEN Styra HLB / Hydrophilic-Lipophilic Balanced Cartridge

SHIMSEN Styra HLB is a hydrophilic-lipophilic balanced cartridge, which is based on styrene-divinylbenzene as the matrix and introduced into the polar functional group containing -N. It has good retention effect on non-polar compounds, and also has a certain retention effect on polar compounds.

• Polystyrene / Divinylbenzene copolymer spherical particles
• Particle size 50 μm, pore size 80 Å, specific surface area 800 m2/g
• Function groups: phenyl, vinyl, pyrrolidone

 

Application:
Environmental monitoring: PAHs, PAEs, phenolic compounds, bisphenol A, triazine herbicides in water and soil;

Food safety testing: analysis of drug residues in animal samples, such as tetracycline drugs, chloramphenicol, sulfonamides, avermectin, macrolides antibiotics, furan drugs and their metabolites, and pesticide residues in plant samples;

Biological samples: analysis of drugs in blood and urine, such as tetracycline drugs, cocaine and its metabolites, morphine and its metabolites, barbiturates, tricyclic drugs, ranitidine, etc.

Part Number	Specification	Pack size
380-00855-03	60 mg / 3 mL	50
380-00855-04	60 mg / 1 mL	100
380-00855-05	6 g / 60 mL	10
380-00855-06	500 mg / 3 mL	50
380-00855-07	500 mg / 6 mL	30
380-00855-08	500 mg / 12 mL	20
380-00855-09	30 mg / 1 mL	100
380-00855-10	200 mg / 6 mL	30
380-00855-11	150 mg / 6 mL	30
380-00855-12	100 mg / 6 mL	30
380-00855-13	1000 mg / 6 mL	30
380-00855-14	1 g / 20 mL	20
380-00855-15	10 mg / 1 mL	100

Back to types of separation modes

Back to top

SHIMSEN Styra MCX / Mixed-Mode Strong Cation Exchange Reversed Phase Cartridge

SHIMSEN Styra MCX uses a polymeric cation-exchange resin that combines the outstanding properties of SHIMSEN Styra HLB with strong cation exchange functionalities. MCX is suitable for the enrichment and purification of basic compounds with pKa 2-10. Retention mechanism: strong cation exchange interaction (primary), non-polar interaction (primary), polar interaction (secondary)

• Polystyrene-divinylbenzene copolymer bonded with sulfonic acid groups
• Particle size 50 μm, pore size 80 Å, specific surface area 800 m2/g
• Function groups: sulfonic acid group, phenyl, vinyl, pyrrolidone
• pKa: <1.0

 

Application:
Food safety testing: melamine analysis; analysis of alkaline drug residues in animal samples, such as sulfonamides, clenbuterol hydrochloride and other drugs; alkaline pesticides in vegetables, fruits and fruit juices, such as carbendazim, thiamethoxam and other fungicides.

Biological samples: analysis of alkaline drugs in blood and urine

Part Number	Specification	Pack Size
380-00853-01	60 mg / 3 mL	50
380-00853-02	6 g / 60 mL	10
380-00853-03	500 mg / 12 mL	20
380-00853-04	30 mg / 1 mL	100
380-00853-05	20 mg / 3 mL	50
380-00853-06	200 mg / 6mL	30
380-00853-07	150 mg / 6 mL	30
380-00853-08	1 mg / 20mL	20

Back to types of separation modes

Back to top

SHIMSEN Styra WCX / Mixed-Mode Weak Cation Exchange Reversed Phase Cartridge

SHIMSEN Styra WCX uses a polymeric cation-exchange resin that combines the outstanding properties of SHIMSEN Styra HLB with weak cation exchange functionalities. WCX is suitable for the enrichment and purification of strong basic compounds with pKa 10 or more. Retention mechanism: weak cation exchange interaction (primary),non-polar interaction (primary), polar interaction (secondary)

• Polystyrene-divinylbenzene copolymer bonded with carboxyl groups
• Particle size 50 μm, pore size 80 Å, specific surface area 800 m2/g
• Function groups: carboxyl, phenyl, vinyl, pyrrolidone
• • pKa: 4.2

 

Application:
Separation and purification of strong basic compounds, such as compounds with quaternary ammonium groups

Part Number	Specification	Pack Size
380-00850-01	60 mg / 3 mL	50
380-00850-02	500 g / 6 mL	30
380-00850-03	500 mg / 3mL	50
380-00850-04	30 mg / 1 mL	100
380-00850-05	150 mg / 6 mL	30
380-00850-06	200 mg / 6 mL	30

Back to types of separation modes

Back to top

SHIMSEN Styra MAX / Mixed-Mode Strong Anion Exchange Reversed Phase Cartridge

SHIMSEN Styra MAX uses a polymeric anion-exchange resin that combines the outstanding properties of SHIMSEN Styra HLB with strong anion exchange functionalities. MAX is suitable for the enrichment and purification of acidic compounds with pKa 2-8. Retention mechanism: anion exchange interaction (primary), non-polar interaction (primary), polar interaction (secondary)

• Polystyrene-divinylbenzene copolymer bonded with quaternary ammonium groups
• Particle size 50 μm, pore size 80 Å, specific surface area 800 m2/g
• Function groups: quaternary ammonium group, phenyl, vinyl, pyrrolidone group
• pKa: completely dissociated

 

Application:
Separation and purification of acidic compounds, such as compounds containing carboxyl and phenolic hydroxyl groups

Part Number	Specification	Pack Size
380-00854-01	60 mg / 3 mL	50
380-00854-02	6 g / 60 mL	10
380-00854-03	500 mg / 6 mL	30
380-00854-04	500 mg / 12 mL	20
380-00854-05	30 mg / 1 mL	100
380-00854-06	150 mg / 6 mL	30
380-00854-07	1000 mg / 6 mL	30
380-00854-08	1 g / 20 mL	20
380-00854-09	200 mg / 3 mL	50

Back to types of separation modes

Back to top

SHIMSEN Styra WAX / Mixed-Mode Weak Anion Exchange Reversed Phase Cartridge

SHIMSEN Styra WAX uses a polymeric anion-exchange resin that combines the outstanding properties of SHIMSEN Styra HLB with weak anion exchange functionalities. WAX is suitable for the enrichment and purification of strong acidic compounds with pKa 1 or less. Retention mechanism: anion exchange interaction (primary), non-polar interaction (primary), polar interaction (secondary)

• Polystyrene-divinylbenzene copolymer bonded with piperazine group
• Particle size 50 μm, pore size 80 Å, specific surface area 800 m2/g
• Function groups: piperazine group, phenyl, vinyl, pyrrolidone
• pKa: 9.5, 5.7 (the pKa value of the conjugated acid of the piperazine group)

 

Application:
Separation and purification of strong acidic compounds, such as compounds containing sulfonic acid groups

Part Number	Specification	Pack Size
380-00852-03	60 mg / 3 mL	50
380-00852-04	500 g / 6 mL	30
380-00852-05	30 mg / 1 mL	100
380-00852-06	150 mg / 6 mL	30

Back to types of separation modes

Back to top

SHIMSEN Styra SI / Normal Phase Silica Cartridge

SHIMSEN Styra SI is based on unbonded silica gel particles. The primary interaction available is strong polar interaction due to the silanol groups. It can be used for enrichment of polar compounds from low-polar solvents or removal of polar impurities from non-polar solvents. It is necessary to prevent moisture absorption before use.

Retention mechanism: Strong polar interaction

• Matrix: Spherical silica particles, particle size 50 μm, pore size 60 Å, specific surface area 500 m2/g
• Function groups: Silicon Hydroxy
• Endcapped: No

 

Application:
Extraction of compounds with polar groups from lipid samples; Adsorption of interfering substances in pesticide residue analysis

Part Number	Specification	Pack Size
380-00856-01	5 mg / 20 mL	20
380-00856-02	500 g / 6 mL	30
380-00856-03	500 mg / 3 mL	50
380-00856-04	2 g / 12 mL	20
380-00856-05	200 mg / 3 mL	50
380-00856-06	2000 mg / 6 mL	30
380-00856-07	10 g / 60 mL	10
380-00856-08	1000 mg / 6 mL	30
380-00856-09	100 mg / 1 mL	100

Back to types of separation modes

Back to top

SHIMSEN Styra FL-PR / Florisil Cartridge

SHIMSEN Styra FL-PR is based on natural Flori silica (MgO • SiO2) and is used for the recovery or cleanup of polar substances from non-polar solvents. This sorbent is high in polarity and can be used to extract polar compounds from non-polar solutions. The larger particle size (200 μm) of the sorbent enables fast flow for large sample volumes and is therefore an attractive alternative to silica if the sample matrix is particularly viscous. FL-PR can not only separate chlorinated insecticides, but also be used in AOAC, EPA and other methods. In addition, FL-PR is an excellent replacement for alumina cartridge when the acidic nature of alumina affects the extraction effect.

Retention mechanism: Polar interaction

• Adsorbent: Magnesium silicate particles with size of 150-200 μm
• Function groups: Silicon Hydroxy

 

Application:
Environmental samples and pesticide residue analysis Food: SHIMSEN Styra FL-PR is suitable for the purification of pesticide residues in crops

Part Number	Specification	Pack Size
380-00862-01	500 mg / 6 mL	30
380-00862-02	500 mg / 3 mL	50
380-00862-03	5 g / 20 mL	20
380-00862-04	2 g / 6 mL	30
380-00862-05	2 g / 12 mL	20
380-00862-06	200 mg / 3 mL	50
380-00862-07	1000 mg / 6 mL	30
380-00862-08	100 mg / 1 mL	100
380-00862-09	10 g / 60 mL	10

Back to types of separation modes

Back to top

SHIMSEN Styra CN / Cyano-bonded Silica Cartridge

SHIMSEN Styra CN exhibits both non-polar and polar interactions with target compound. It is a good choice when sorbents such as C18, C8 or Si do not provide the desired selectivity.

Retention mechanism: Polar interaction and non-polar interaction

• Matrix: Particle size 50 μm, pore size 60 Å
• Spherical silica particles with a specific surface area of 500 m2/g
• Function groups: Cyanopropyl
• Endcapped: Yes
• Carbon load (C%): 6.5%

 

Application:
Pesticides, drugs and their metabolites in aqueous samples.

Part Number	Specification	Pack Size
380-00864-01	500 mg / 3 mL	50
380-00864-02	5 g / 20 mL	20
380-00864-03	200 mg / 3 mL	50
380-00864-04	2 g / 12 mL	20
380-00864-05	1000 mg / 6mL	30
380-00864-06	100 mg / 1 mL	100
380-00864-07	10 g / 60 mL	10

Back to types of separation modes

Back to top

SHIMSEN Styra AL-N / Neutral Alumina Cartridge

SHIMSEN Styra AL-N is packed with neutral alumina (Al2O3) to retain or remove polar compounds. Similar to silica gel, neutral Al2O3 is a strongly polar sorbent. However, the neutral alumina tends to be slightly more stable under high pH conditions than silica gel. The neutralized surface allows interaction with compounds whose heteroatoms are electronegative (e.g., O, P, S) or with an electron-rich, highly aromatic structure.

Retention mechanism: Lewis acid-base interaction, polar interaction and ion exchange interaction

• Adsorbent: Neutral AI2O3 particles with size of 125 μm
• pH ：7.5

 

Application:
SHIMSEN Styra AL-N is used to extract polar or non-polar compounds from both aqueous and non-aqueous solutions, such as sample preparation of malachite green and crystal violet in aquatic products.

Part Number	Specification	Pack Size
380-00865-01	500 mg / 6 mL	30
380-00865-02	500 mg / 3 mL	50
380-00865-03	5 g / 20 mL	20
380-00865-04	250 mg / 3 mL	50
380-00865-05	200 mg / 3 mL	50
380-00865-06	2 g / 12 mL	20
380-00865-07	1000 mg / 6 mL	30
380-00865-08	100 mg / 1 mL	100
380-00865-09	10 g / 60 mL	10
380-00865-10	3 g / 12 mL	20
380-00865-11	1000 mg / 3 mL	50
380-00865-12	60 mg / 3 mL	50

Back to types of separation modes

Back to top

SHIMSEN Styra AL-A / Acidic Alumina Cartridge

SHIMSEN Styra AL-A is packed with acidic alumina (Al2O3) to retain or remove polar compounds and acidic compounds. Acidic alumina is a typical Lewis acid with two electrons missing from the aluminum atom. Acid treatment enhances the Lewis acid property of alumina, and results in a weak cationic property, allowing strong interaction with electron-rich compounds and retention of polar compounds and anionic compounds.

Retention mechanism: Lewis acid-base interaction, polar interaction and ion exchange interaction

• Adsorbent: Acidic AI2O3 particles with size of 125 μm
• pH ：4.5

 

Application:
Retention of polar compounds and anionic compounds.

Part Number	Specification	Pack size
380-00867-01	500 mg / 6mL	30
380-00867-02	500 mg / 3 mL	50
380-00867-03	5 g / 20 mL	20
380-00867-04	2 g / 12 mL	20
380-00867-05	1000 mg / 6 mL	30
380-00867-06	100 mg / 1 mL	100
380-00867-07	10 g / 60 mL	10

Back to types of separation modes

Back to top

SHIMSEN Styra AL-B / Basic Alumina Cartridge

SHIMSEN Styra AL-B is packed with basic alumina (Al2O3) to retain or remove polar compounds and basic compounds. Basic alumina has anionic properties and cation exchange function, and tends to retain compounds with positive charges or hydrogen bonds. In addition, it can be used to retain electronic samples, such as neutral amines.

Retention mechanism: Lewis acid-base interaction, polar interaction and ion exchange interaction

• Adsorbent: Basic AI2O3 particles with size of 125 μm
• pH ：10

 

Application:
Retention of polar compounds and anionic compounds. It is suitable for the detection of Sudan red in hot pepper.

Part Number	Specification	Pack size
380-00866-01	500 mg / 3 mL	50
380-00866-02	5 g / 20 mL	20
380-00866-03	2 g / 12 mL	20
380-00866-04	1000 mg / 3 mL	50
380-00866-05	1000 mg / 6 mL	30
380-00866-06	100 mg / 1 mL	100
380-00866-07	10 g / 60 mL	10

Back to types of separation modes

Back to top

SHIMSEN Styra NH2 / Amino Bonded Silica Cartridge

SHIMSEN Styra NH2 is an amino bonded silica cartridge and can act either as a weak anion exchanger (aqueous solutions) or polar phase sorbent (non-polar organic solutions). When conditioned with a non-polar solvent, e.g., hexane, it tends to form hydrogen bond with any molecule containing -OH, -NH, or -SH functional groups. In an aqueous environment with pH 7.8 or less, it can function as a weak anion exchanger.

Retention mechanism: Polar interaction and weak anion exchange

• Matrix: Particle size 50 μm, pore size 60 Å
• Spherical silica particles with a specific surface area of 500 m2/g
• Function groups: Cyanopropyl
• Endcapped: No
• Carbon load (C%): 3.5%
• pKa: 9.8 (the pKa of its conjugated acid)

 

Application:
It can be used to separate structural isomers; extract compounds with polar groups from lipid samples; remove polar compounds (such as carbohydrates, pigments), organic acids, and phenols, etc. in pesticide residue analysis.

 

Part Number	Specification	Pack Size
380-00861-01	6 g / 60 mL	10
380-00861-02	500 mg / 6 mL	30
380-00861-03	500 mg / 3 mL	50
380-00861-04	5 g / 20 mL	20
380-00861-05	200 mg / 3 mL	50
380-00861-06	2 g / 12 mL	20
380-00861-07	1000 mg / 6 mL	30
380-00861-08	100 mg / 1 mL	100
380-00861-09	10 g / 60 mL	10

Back to types of separation modes

Back to top

SHIMSEN Styra PSA / Ethylenediamino Bonded Silica Cartridge

SHIMSEN Styra PSA is based on silica gel particles bonded with ethylenediamino-N-propyl which contain two different amino―the pKa of one is 10.1, and other is 10.9. It is similar to NH2 cartridge but has stronger ion exchange functionalities. At the same time, PSA can be used to extract metal ion due to the chelation with metal ion.

Retention mechanism: Weak anion exchange, polar interaction, chelation

• Matrix: Particle size 50 μm, pore size 60 Å
• Spherical silica particles with a specific surface area of 500 m2/g
• Function groups: Ethylenediamine-N-propyl
• Endcapped: No
• Carbon load (C%): 7%
• pKa: 10.1 and 10.9 (the pKa of its conjugated acid)

 

Application:
It can be used to separate structural isomers; extract compounds with polar groups from lipid samples, and remove polar compounds (such as carbohydrates, pigments), organic acids, phenols, etc. in pesticide residue analysis.

 

Part Number	Specification	Pack Size
380-00859-01	500 mg / 6 mL	30
380-00859-02	500 mg / 3 mL	50
380-00859-03	5 g / 20 mL	20
380-00859-04	200 mg / 3 mL	50
380-00859-05	2 g / 12 mL	20
380-00859-06	1000 mg / 6 mL	30
380-00859-07	100 mg / 1 mL	100
380-00859-08	10 g / 60 mL	10

Back to types of separation modes

Back to top

SHIMSEN Styra C18 / Octadecyl Bonded Silica Cartridge

SHIMSEN Styra C18 is an octadecyl bonded silica cartridge and can retain target compounds by non-polar interaction. Highly endcapping of this sorbent inhibits cation exchange interaction caused by silanol groups, resulting in less adsorption of basic compounds.
It is a good choice when non-polar interaction is needed.

Retention mechanism: Strong non-polar interaction

• Matrix: Particle size 50 μm, pore size 60 Å
• Spherical silica particles with a specific surface area of 500 m2/g
• Function groups: Octadecyl
• Endcapped: Yes
• Carbon load (C%): 17%

 

Application:
Extraction of organic pollutants from water: PAHs, PAEs, PCBs, pesticides,herbicides, phenols, etc.;
Life sciences: extraction of drugs and their metabolites from plasma, serum, and urine;
Extraction of pesticide and veterinary drug residues from food;
Animal and plant extracts: aromatic oils, fat-soluble vitamins, water-soluble vitamins, carbohydrates, organic acids, steroids, etc.;
Desalination of biological macromolecules.

 

Part Number	Specification	Pack Size
380-00872-01	60 mg / 3 mL	50
380-00872-02	500 mg / 6 mL	30
380-00872-03	500 mg / 3 mL	50
380-00872-04	50 mg / 1 mL	100
380-00872-05	5 g / 20 mL	20
380-00872-06	250 mg / 3 mL	50
380-00872-07	200 mg / 6 mL	30
380-00872-08	200 mg / 3 mL	50
380-00872-09	2 g / 12 mL	20
380-00872-10	100 mg / 6 mL	30
380-00872-11	100 mg / 3 mL	50
380-00872-12	1000 mg / 6 mL	30
380-00872-13	100 mg / 1 mL	100
380-00872-14	10 g / 60 mL	10
380-00872-15	300 mg / 3mL	50

Back to types of separation modes

Back to top

SHIMSEN Styra C18-U / Octadecyl Bonded Silica Cartridge

SHIMSEN Styra C18-U is an octadecyl bonded silica cartridge and can retain target compounds by non-polar
interaction. The phase is non-endcapped which enhances retention of polar compounds.

Retention mechanism: Non-polar interaction (primary) , polar interaction (secondary)

• Matrix: Particle size 50 μm, pore size 60 Å
• Spherical silica particles with a specific surface area of 500 m2/g
• Function groups: Octadecyl
• Endcapped: No
• Carbon load (C%): 17%

 

Application:
C18-U is a non-endcapped version of the octadecyl bonded phases. The residual silanols on the surface of the silica gel matrix have polar interaction with polar compounds, which can enhance retention of polar compounds, especially amine compounds (such as tetracycline drugs), and is suitable for the extraction of polar and non-polar compounds.

Part Number	Specification	Pack Size
380-00876-01	60 mg / 3 mL	50
380-00876-02	500 mg / 20 mL	20
380-00876-03	500 mg / 6 mL	30
380-00876-04	500 mg / 3 mL	50
380-00876-05	50 mg / 1 mL	100
380-00876-06	5 g / 20 mL	20
380-00876-07	200 mg / 3 mL	50
380-00876-08	2 g / 12 mL	20
380-00876-09	1000 mg / 6 mL	30
380-00876-10	100 mg / 1 mL	100
380-00876-11	10 g / 60 mL	10

Back to types of separation modes

Back to top

SHIMSEN Styra C8 / Octyl Bonded Silica Cartridge

SHIMSEN Styra C8 is an octyl bonded silica cartridge and has weaker non-polar interation compared to C18. C8 is an excellent replacement for C18 when highly hydrophobic compounds are too strongly retained for effective elution. Highly endcapping of this sorbent inhibits secondary interaction caused by silanol groups, resulting in less adsorption of basic compounds.

Retention mechanism: Moderate non-polar interaction

• Matrix: Particle size 50 μm, pore size 60 Å
• Spherical silica particles with a specific surface area of 500 m2/g
• Function groups: Octyl
• Endcapped: Yes
• Carbon load (C%): 11%

Application:
It is suitable for retention of moderate polar compounds, drugs and their metabolites from biological samples, and extraction of peptides from biological samples, etc.

 

Part Number	Specification	Pack Size
380-00870-01	500 mg / 6 mL	30
380-00870-02	500 mg / 3 mL	50
380-00870-03	5 g / 20 mL	20
380-00870-04	200 mg / 3 mL	50
380-00870-05	2 g / 12 mL	20
380-00870-06	1000 mg / 6 mL	30
380-00870-07	100 mg / 1 mL	100
380-00870-08	10 g / 60 mL	10

Back to types of separation modes

Back to top

SHIMSEN Styra C2 / Ethyl Bonded Silica Cartridge

SHIMSEN Styra C2 is an ethyl bonded silica cartridge and has weaker non-polar interation compared to C8. It is an excellent replacement for C8 when highly hydrophobic compounds are too strongly retained for effective elution. Highly endcapping of this sorbent inhibits secondary interactions caused by silanol groups, resulting in less adsorption of basic compounds. It is an excellent replacement for C8 and C18 when analytes are too strongly retained for effective elution.

Retention mechanism: Non-polar interaction (primary) and polar interaction (secondary)

• Matrix: Particle size 50 μm, pore size 60 Å
• Spherical silica particles with a specific surface area of 500 m2/g
• Function groups: Ethyl
• Endcapped: Yes
• Carbon load (C%): 2.7%

 

Application:
The polarity of C2 group is slightly lower than CN group. SHIMSEN Styra C2 is usually used to extract drugs from plasma and serum samples.
Extraction of drugs and metabolites from blood samples.

 

Part Number	Specification	Pack Size
380-00871-01	500 mg / 3 mL	50
380-00871-02	5 g / 20 mL	20
380-00871-03	2 g / 12 mL	20
380-00871-04	1000 mg / 6 mL	30
380-00871-05	100 mg / 1 mL	100
380-00871-06	10 g / 60 mL	10

Back to types of separation modes

Back to top

SHIMSEN Styra Ph / Phenyl Bonded Silica Cartridge

SHIMSEN Styra GCB is packed with planarized graphitized carbon, mainly used to remove pigment components from crops. The polarity and ion exchange property of this cartridge are used to purify all kinds of sample substrates. The typical application of this cartridge is the analysis of pesticide residues in agricultural products. Plant samples often contain various pigments, such as chlorophyll, lutein, carotenoids, etc, which need to be removed in order to eliminate its interference with the analysis results and experimental instruments.

Retention mechanism: Surface adsorption

• Adsorbent: graphitized carbon with lamellar structure
• Particle size: 120-400 mesh

 

Application:
Sample cleanup in pesticide residue analysis of vegetables, fruits, tea and plant products.

Product number	Specification	Pack size
380-00869-01	500 mg / 3 mL	50
380-00869-02	500 mg / 6 mL	30
380-00869-03	2 g / 12 mL	20
380-00869-04	250 mg / 3 mL	50
380-00869-05	200 mg / 6 mL	30
380-00869-06	1 g / 6 mL	30
380-00869-07	125 mg / 3 mL	50
380-00869-08	100 mg / 3 mL	50
380-00869-09	100 mg / 1 mL	100

Back to types of separation modes

Back to top

SHIMSEN Styra SCX / Strong Cation Exchange Cartridge

SHIMSEN Styra SCX is a propyl benzenesulfonate bonded silica cartridge and has strong cation exchange functionalities. The presence of the benzene ring in the functional group increases its nonpolar characteristics. Besides, SHIMSEN Styra SCX is suitable for the separation of basic compounds.

Retention mechanism: Strong cation exchange interaction and reverse phase interaction (primary); - electron pair interaction (secondary)

• Matrix: Particle size 50 μm, pore size 60 Å
• Spherical silica particles with a specific surface area of 500 m2/g
• Function groups: P-propylbenzene sulfonic acid
• Endcapped: No
• Carbon load (C%): 10.9%
• pKa ：<1.0

Application:
Enrichment and purification of alkaline compounds from aqueous samples, biological fluids and organic phases.

Product Number	Specification	Pack Size
380-00857-01	60 mg / 3 mL	50
380-00857-02	500 mg / 6 mL	30
380-00857-03	500 mg / 3 mL	50
380-00857-04	5 g / 20 mL	20
380-00857-05	2 g / 12 mL	20
380-00857-06	100 mg / 12 mL	20
380-00857-07	1000 mg / 6 mL	30
380-00857-08	100 mg / 1 mL	100
380-00857-09	10 g / 60 mL	10

Back to types of separation modes

Back to top

SHIMSEN Styra SAX / Strong Anion Exchange Cartridge

SHIMSEN Styra SAX is a trimethylaminopropyl-bonded silica cartridge and has strong anion exchange functionalities.It can provide selective retention for anionic organic compounds (such as compounds containing carboxyl groups and phenolic hydroxyl groups). The non-polar interactions are weak due to its short carbon chain on the quaternary ammonium group.

Retention mechanism: Strong anion exchange interaction (primary), nonpolar interaction (secondary)

• Matrix: Particle size 50 μm, pore size 60 Å
• Spherical silica particles with a specific surface area of 500 m2/g
• Function groups: Trimethylaminopropyl
• Endcapped: No
• Carbon load (C%): 7.5%
• pKa: Completely dissociated

 

Applications:

• SHIMSEN Styra SAX is generally used for the retention of weak anionic compounds containing carboxylic acid groups.
• Enrichment and purification of anionic organic compounds from aqueous samples, biological fluids and organic phases.

 

Product Number	Specifications	Pack size
380-00858-01	500 mg / 6 mL	30
380-00858-02	500 mg / 3 mL	50
380-00858-03	5 g / 20 mL	20
380-00858-04	2 g / 12 mL	20
380-00858-05	100 mg / 1 mL	100
380-00858-06	10 g / 60 mL	10
380-00858-07	1000mg / 6ml	30

Back to types of separation modes

Back to top

SHIMSEN Styra GCB / Graphitized Carbon Cartridge

SHIMSEN Styra GCB is packed with planarized graphitized carbon, mainly used to remove pigment components from crops. The polarity and ion exchange property of this cartridge are used to purify all kinds of sample substrates. The typical application of this cartridge is the analysis of pesticide residues in agricultural products. Plant samples often contain various pigments, such as chlorophyll, lutein, carotenoids, etc, which need to be removed in order to eliminate its interference with the analysis results and experimental instruments.

Retention mechanism: Surface adsorption

• Adsorbent: graphitized carbon with lamellar structure
• Particle size: 120-400 mesh

 

Application:
Sample cleanup in pesticide residue analysis of vegetables, fruits, tea and plant products.

Product number	Specification	Pack size
380-00869-01	500 mg / 3 mL	50
380-00869-02	500 mg / 6 mL	30
380-00869-03	2 g / 12 mL	20
380-00869-04	250 mg / 3 mL	50
380-00869-05	200 mg / 6 mL	30
380-00869-06	1 g / 6 mL	30
380-00869-07	125 mg / 3 mL	50
380-00869-08	100 mg / 3 mL	50
380-00869-09	100 mg / 1 mL	100

Back to types of separation modes

Back to top

SHIMSEN Styra GCB / PSA Graphitized Carbon Composite PSA Cartridge
SHIMSEN Styra GCB / NH2 Graphitized Carbon Composite Amino Cartridge

SHIMSEN Styra GCB/PSA and SHIMSEN Styra GCB/NH2 are mixed sorbent SPE cartridges consisting of graphitized carbon particles (GCB) and silica gel particles bonded with PSA or NH2 group. The GCB can remove pigments, while PSA and NH2 can remove anionic compounds. They are mainly used for the analysis of multi-pesticide residues in food samples.

Retention mechanism: Surface adsorption and anion exchange founction

Application:
They are widely used in the analysis of multiple pesticide residues in food.

SHIMSEN Styra GCB/PSA:

Part Number	Specification	Pack Size
380-00868-01	500 mg / 500 mg / 6 mL	30
380-00868-02	500 mg / 500 mg /20 mL	20
380-00868-03	250 mg / 250 mg / 6 mL	30

SHIMSEN Styra GCB/NH2:

Part Number	Specification	Pack Size
380-00868-04	500 mg / 500 mg / 6 mL	30
380-00868-05	500 mg / 500 mg / 20 mL	20
380-00868-06	250 mg / 250 mg / 6 mL	30

Back to types of separation modes

Back to top

SHIMSEN Styra GAC / Activated Carbon Cartridge

SHIMSEN Styra GAC is packed with activated carbon particles and used to analyse pesticides, herbicides and acrylamide in aqueous samples.

Retention mechanism: Surface adsorption

Part number	Specification	Pack size
380-00874-01	500 mg / 6 mL	30
380-00874-02	2 g / 12 mL	20
380-00874-03	250 mg / 3 mL	50

Back to types of separation modes

Back to top

SHIMSEN Styra SAX/PSA / Mixed-Mode Strong Anion Exchange Normal Phase Cartridge

SHIMSEN Styra SAX/PSA is packed with 2 layers of anion exchange packing to enhance the removal of anionic impurities in samples. It is widely used in the analysis of multiple pesticide residues in food.

Part number	Specification	Pack size
380-00877-05	250 mg / 250 mg / 6 mL	30

Back to types of separation modes

Back to top

SHIMSEN Styra Glass / Glass Cartridge

SHIMSEN Styra Glass is developed specifically for high-purity extraction and suitable for detection on ppt level. The inert glass tube completely eliminates contamination from plasticizers, such as phthalates. The sorbent and the specially cleaned sieve plate used in SHIMSEN Styra glass cartridge are all high-grade to ensure stability and repeatability.

Part Number	Packing Media	Specification	Pack Size
380-00855-01	HLB	500 mg / 6 mL	30
380-00873-01		200 mg / 6 mL	30
380-00873-02	SI	500 mg / 6 mL	30
380-00873-03		2000 mg / 6 mL	30
380-00873-04	GCB/NH2	500 mg / 500 mg / 6 mL	30
380-00873-05	C18	500 mg / 6 mL	30
380-00873-06	FL-PR	1 g / 6 mL	30
380-00873-07		500 mg / 6 mL	30
380-00873-08	PSA/SI	500 mg / 500 mg / 6 mL	30
380-00873-09	SI	1 g / 6 mL	30
380-00873-11	PSA	1 g / 6 mL	30
380-00873-12		500 mg / 6 mL	30
380-00873-13	AL-N	1000 mg / 6 mL	30

Back to types of separation modes

Back to top

SHIMSEN Styra Na2SO4 / Dehydration / Drying Cartridge

SHIMSEN Styra Na2SO4 is a special dehydrating column filled with anhydrous sodium sulfate.

Part Number	Specification	Pack Size
380-00875-01	5 g / 6 mL	30
380-00875-02	500 mg / 3 mL	50
380-00875-03	4 g / 6 mL	30
380-00875-04	3 g / 6 mL	30
380-00875-05	2 g / 6 mL	30
380-00875-06	1 g / 6 mL	30
380-00875-07	1 g / 3 mL	50

Back to types of separation modes

Back to top