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O-Glycan Analysis
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Description:
STREAMLINED, RAPID and RELIABLE HPLC and LC-MS-Ready SAMPLE PREPARATION of O-GLYCANS EZGlyco® O-Glycan Prep Kit provides a streamlined, rapid and reliable O-glycan sample preparation. The complete kit includes all reagents to release, enrich, label and purify O-glycans for HPLC and LC-MS analysis. The kit adopts a unique O-glycan releasing method “Eliminative Oximation” using a high reactive amine and an organic superbase, which yields high recovery and less degradation (peeling) of released O-glycans. Applications include characterization of O-glycosylation of recombinant glycoproteins in upstream R&D, quality control and process control. It is also suited for O-glycan biomarker discovery in biological samples such as mucins, serum and exosome, and O-glycan analysis of functional foods such as dairy products. Content: Protocol Glycan Release Reagent A Glycan Release Reagent B Glycan Capturing Beads Filter Column 2-Aminobezamide Reducing Reagent Cleanup Column O-GLYCAN SAMPLE PREP IN 5 HOURS O-Glycan sample preparation is completed in 5 hours with its unique O-glycan releasing reagents. Thus, HPLC/LC-MS analysis is achieved on the same day. HIGH RECOVERY A combination of the unique O-glycan releasing reagent and the new O-Glycan Capturing Beads enables high recovery of released O-glycans from various glycoproteins. MINIMIZE “PEELING” Glycan releasing reagents minimize decomposition of O-glycans (peeling), resulting in O-glycan characterization with higher accuracy. EASY AND SAFE OPERATION With a simple protocol to follow, O-glycan sample preparation is completed without any special laboratory equipment and toxic reagents.
OUT OF STOCKSTREAMLINED, RAPID and RELIABLE HPLC and LC-MS-Ready SAMPLE PREPARATION of O-GLYCANS EZGlyco® O-Glycan Prep Kit provides a streamlined, rapid and reliable O-glycan sample preparation. The complete kit includes all reagents to release, enrich, label and purify O-glycans for HPLC and LC-MS analysis. The kit adopts a unique O-glycan releasing method “Eliminative Oximation” using a high reactive amine and an organic superbase, which yields high recovery and less degradation (peeling) of released O-glycans. Applications include characterization of O-glycosylation of recombinant glycoproteins in upstream R&D, quality control and process control. It is also suited for O-glycan biomarker discovery in biological samples such as mucins, serum and exosome, and O-glycan analysis of functional foods such as dairy products. Content: Protocol Glycan Release Reagent A Glycan Release Reagent B Glycan Capturing Beads Filter Column 2-Aminobezamide Reducing Reagent Cleanup Column O-GLYCAN SAMPLE PREP IN 5 HOURS O-Glycan sample preparation is completed in 5 hours with its unique O-glycan releasing reagents. Thus, HPLC/LC-MS analysis is achieved on the same day. HIGH RECOVERY A combination of the unique O-glycan releasing reagent and the new O-Glycan Capturing Beads enables high recovery of released O-glycans from various glycoproteins. MINIMIZE “PEELING” Glycan releasing reagents minimize decomposition of O-glycans (peeling), resulting in O-glycan characterization with higher accuracy. EASY AND SAFE OPERATION With a simple protocol to follow, O-glycan sample preparation is completed without any special laboratory equipment and toxic reagents.