EZGlyco™ O-Glycan Prep Kit

STREAMLINED, RAPID and RELIABLE HPLC and LC-MS-Ready SAMPLE PREPARATION of O-GLYCANS 

EZGlyco® O-Glycan Prep Kit provides a streamlined, rapid and reliable O-glycan sample preparation. The complete kit includes all reagents to release, enrich, label and purify O-glycans for HPLC and LC-MS analysis. The kit adopts a unique O-glycan releasing method “Eliminative Oximation” using a high reactive amine and an organic superbase, which yields high recovery and less degradation (peeling) of released O-glycans. Applications include characterization of O-glycosylation of recombinant glycoproteins in upstream R&D, quality control and process control. It is also suited for O-glycan biomarker discovery in biological samples such as mucins, serum and exosome, and O-glycan analysis of functional foods such as dairy products.

Content:

• Protocol
• Glycan Release Reagent A
• Glycan Release Reagent B
• Glycan Capturing Beads
• Filter Column
• 2-Aminobezamide
• Reducing Reagent
• Cleanup Column

O-GLYCAN SAMPLE PREP IN 5 HOURS

O-Glycan sample preparation is completed in 5 hours with its unique O-glycan releasing reagents. Thus, HPLC/LC-MS analysis is achieved on the same day.

HIGH RECOVERY

A combination of the unique O-glycan releasing reagent and the new O-Glycan Capturing Beads enables high recovery of released O-glycans from various glycoproteins.

MINIMIZE “PEELING”

Glycan releasing reagents minimize decomposition of O-glycans (peeling), resulting in O-glycan characterization with higher accuracy.

EASY AND SAFE OPERATION

With a simple protocol to follow, O-glycan sample preparation is completed without any special laboratory equipment and toxic reagents.