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n-glycan

EZGlyco™ mAb-N Kit with 2-AB

Part Number: APASBC-BSX4410Z

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Type: N-Glycan Kit

Take Note: Additional shipping charges may be applied to your order at time of shipment

Dangerous Goods: Yes

Shipment Origin: Direct shipment from Japan

Lead Time: 2-3 Weeks

HPLC-Ready N-glycan Preparation

The EZGlyco™ mAb-N Kit with 2-AB is a complete kit which includes the enzyme, reagents, and accessories necessary for completion of HPLC-ready N-glycan preparation in less than 2 hours of handling time. It also provides traditional 2-aminobenzamide labeling designed with a non-toxic reductant.

The EZGlyco™ mAb-N Kit with 2-AB is a complete kit which includes the enzyme, reagents, and accessories necessary for completion of HPLC-ready N-glycan preparation in less than 2 hours of handling time. It also provides traditional 2-aminobenzamide labeling designed with a non-toxic reductant.

Contents

  • Antibody Capturing Column (Qty 10)
  • Silicone Bottom Cap (Qty 10)
  • Antibody Capturing Solution (Qty 1)
  • Washing Buffer (Qty 1)
  • Glycan Release Enhancer (Qty 1)
  • PNGase F Solution (Qty 1)
  • 2-Aminobenzamide (Qty 3)
  • Reducing Reagent (Qty 3)
  • Cleanup Column (Qty 10)
  • Instruction Manual (Qty 1)

 

BS-X4410Z

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o-glycan
EZGlyco™ O-Glycan Prep Kit

Part No.: APASBC-BS41601Z

Glycan Analysis Kits

STREAMLINED, RAPID and RELIABLE HPLC and LC-MS-Ready SAMPLE PREPARATION of O-GLYCANS    EZGlyco® O-Glycan Prep Kit provides a streamlined, rapid and reliable O-glycan sample preparation. The complete kit includes all reagents to release, enrich, label and purify O-glycans for HPLC and LC-MS analysis. The kit adopts a unique O-glycan releasing method “Eliminative Oximation” using a high reactive amine and an organic superbase, which yields high recovery and less degradation (peeling) of released O-glycans. Applications include characterization of O-glycosylation of recombinant glycoproteins in upstream R&D, quality control and process control. It is also suited for O-glycan biomarker discovery in biological samples such as mucins, serum and exosome, and O-glycan analysis of functional foods such as dairy products. Content: Protocol Glycan Release Reagent A Glycan Release Reagent B Glycan Capturing Beads Filter Column 2-Aminobezamide Reducing Reagent Cleanup Column   O-GLYCAN SAMPLE PREP IN 5 HOURS O-Glycan sample preparation is completed in 5 hours with its unique O-glycan releasing reagents. Thus, HPLC/LC-MS analysis is achieved on the same day. HIGH RECOVERY A combination of the unique O-glycan releasing reagent and the new O-Glycan Capturing Beads enables high recovery of released O-glycans from various glycoproteins. MINIMIZE “PEELING” Glycan releasing reagents minimize decomposition of O-glycans (peeling), resulting in O-glycan characterization with higher accuracy. EASY AND SAFE OPERATION With a simple protocol to follow, O-glycan sample preparation is completed without any special laboratory equipment and toxic reagents.

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