UHPLC and HPLC Columns

Fully Porous Hybrid Particle Based Column Series Excellent stability and performance could be achieved under a wide range of LC conditions with Shim-pack Scepter™ LC columns, which are the next generation organic silica hybrid based columns. With different chemistry characteristics, Shim-pack Scepter columns are effective for method development/scouting with suitability for use in a wide variety of applications. With different particle sizes (1.9 μm, 3 μm, 5 μm) and different column dimensions, Shim-pack Scepter LC columns are fully scalable between UHPLC, HPLC and preparative LC making method transfer seamless between different laboratory instrumentation. Method Scouting Utilize excellent stability & performance under a wide range of LC conditions With excellent stability under a wide range of LC conditions, Shim-pack Scepter LC columns are effective for method scouting combining mobile phase pH and organic modifier. Comparison of Chromatograms using Gradient condition with Acetonitrile:  

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Shim-pack Bio HIC Column is a hydrophobic interaction chromatography (HIC) column packed with butyl bonded hydrophilic nonporous polymer particles (4 μm). HIC is suitable for the separation of anlaytes with slightly different hydrophobicity, such as antibody-drug conjugate (ADC) with different drug-antibody ratios (DAR). Shim-pack Bio HIC can be used for the analysis of DAR of ADC with relatively low pressure and high resolution. Need for DAR Analysis in ADCs ADCs consist of lysine and cysteine residues of a monoclonal antibody (mAb) and small-molecule drugs (Fig.1). However, since mAbs contain multiple amino acid residues involved in their binding, the mAbs are known to form heterogeneous ADCs with different binding numbers and binding sites. These heterogeneities can affect a drug's efficacy and safety, making analysis of the drug-antibody ratio (DAR) essential to determine the quality characteristics of ADCs. In addition, maintaining the distribution of DAR within a certain range enables quality assurance in ADC manufacturing.   Excellent Reproducibility to Help Calculate DAR Reproducibility of area values obtained from repeat injections (n= 6) of cysteine-conjugated ADC samples and an example of an automated DAR calculation report are shown below. (DAR can be calculated automatically using LabSolutions DB/CS custom reporting.) The Shim-pack BIO HIC, developed for biopharmaceutical analysis, makes it possible to detect slight differences in hydrophobicity of small-molecule drugs and perform rapid DAR analysis with high resolution. Download flyer

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Shim-pack Scepter C4-300 is wide-pore organic silica hybrid reversed phase columns recommended for the separation of proteins such as monoclonal antibodies and mid-sized molecules such as oligonucleic acids and peptides that may not be retained on 100-120Å pore size columns due to size-exclusion effects. The organic silica hybrid base material is highly stable even at high temperatures under acidic and basic mobile phase conditions. Pore sizes are optimized for large molecular weight compounds and dispersed uniformly in the column, resulting in symmetrical peak shapes and increased resolution of antibodies and nucleic acids compared to a smaller pore size column. Shim-pack Scepter C4-300 is also effective for high-sensitivity LC/MS analysis, producing good peak shapes even under weak ionpairing conditions using a formic acid mobile phase Peptide Analysis Example Shim-pack Scepter C18-300 and C4-300 made with 300 Å (30 nm) pore size packing material. The larger pore size is recommended for the analysis of mid-sized and large-sized molecules with molecular weights above 5000 to enable retention and avoid size-exclusion effects. This analysis of a mixed sample of four different peptides and proteins demonstrated good peak shapes and favorable separation of insulin and ribonuclease A due to the pore size that was large enough to allow proper diffusion of these large molecular weight compounds.  

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Shim-pack Scepter C4-300 is wide-pore organic silica hybrid reversed phase columns recommended for the separation of proteins such as monoclonal antibodies and mid-sized molecules such as oligonucleic acids and peptides that may not be retained on 100-120Å pore size columns due to size-exclusion effects. The organic silica hybrid base material is highly stable even at high temperatures under acidic and basic mobile phase conditions. Pore sizes are optimized for large molecular weight compounds and dispersed uniformly in the column, resulting in symmetrical peak shapes and increased resolution of antibodies and nucleic acids compared to a smaller pore size column. Shim-pack Scepter C4-300 is also effective for high-sensitivity LC/MS analysis, producing good peak shapes even under weak ionpairing conditions using a formic acid mobile phase Peptide Analysis Example Shim-pack Scepter C18-300 and C4-300 made with 300 Å (30 nm) pore size packing material. The larger pore size is recommended for the analysis of mid-sized and large-sized molecules with molecular weights above 5000 to enable retention and avoid size-exclusion effects. This analysis of a mixed sample of four different peptides and proteins demonstrated good peak shapes and favorable separation of insulin and ribonuclease A due to the pore size that was large enough to allow proper diffusion of these large molecular weight compounds.  

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Shim-pack Scepter C4-300 is wide-pore organic silica hybrid reversed phase columns recommended for the separation of proteins such as monoclonal antibodies and mid-sized molecules such as oligonucleic acids and peptides that may not be retained on 100-120Å pore size columns due to size-exclusion effects. The organic silica hybrid base material is highly stable even at high temperatures under acidic and basic mobile phase conditions. Pore sizes are optimized for large molecular weight compounds and dispersed uniformly in the column, resulting in symmetrical peak shapes and increased resolution of antibodies and nucleic acids compared to a smaller pore size column. Shim-pack Scepter C4-300 is also effective for high-sensitivity LC/MS analysis, producing good peak shapes even under weak ionpairing conditions using a formic acid mobile phase Peptide Analysis Example Shim-pack Scepter C18-300 and C4-300 made with 300 Å (30 nm) pore size packing material. The larger pore size is recommended for the analysis of mid-sized and large-sized molecules with molecular weights above 5000 to enable retention and avoid size-exclusion effects. This analysis of a mixed sample of four different peptides and proteins demonstrated good peak shapes and favorable separation of insulin and ribonuclease A due to the pore size that was large enough to allow proper diffusion of these large molecular weight compounds.  

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Shim-pack Scepter C4-300 is wide-pore organic silica hybrid reversed phase columns recommended for the separation of proteins such as monoclonal antibodies and mid-sized molecules such as oligonucleic acids and peptides that may not be retained on 100-120Å pore size columns due to size-exclusion effects. The organic silica hybrid base material is highly stable even at high temperatures under acidic and basic mobile phase conditions. Pore sizes are optimized for large molecular weight compounds and dispersed uniformly in the column, resulting in symmetrical peak shapes and increased resolution of antibodies and nucleic acids compared to a smaller pore size column. Shim-pack Scepter C4-300 is also effective for high-sensitivity LC/MS analysis, producing good peak shapes even under weak ionpairing conditions using a formic acid mobile phase Peptide Analysis Example Shim-pack Scepter C18-300 and C4-300 made with 300 Å (30 nm) pore size packing material. The larger pore size is recommended for the analysis of mid-sized and large-sized molecules with molecular weights above 5000 to enable retention and avoid size-exclusion effects. This analysis of a mixed sample of four different peptides and proteins demonstrated good peak shapes and favorable separation of insulin and ribonuclease A due to the pore size that was large enough to allow proper diffusion of these large molecular weight compounds.  

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Shim-pack Scepter C4-300 is wide-pore organic silica hybrid reversed phase columns recommended for the separation of proteins such as monoclonal antibodies and mid-sized molecules such as oligonucleic acids and peptides that may not be retained on 100-120Å pore size columns due to size-exclusion effects. The organic silica hybrid base material is highly stable even at high temperatures under acidic and basic mobile phase conditions. Pore sizes are optimized for large molecular weight compounds and dispersed uniformly in the column, resulting in symmetrical peak shapes and increased resolution of antibodies and nucleic acids compared to a smaller pore size column. Shim-pack Scepter C4-300 is also effective for high-sensitivity LC/MS analysis, producing good peak shapes even under weak ionpairing conditions using a formic acid mobile phase Peptide Analysis Example Shim-pack Scepter C18-300 and C4-300 made with 300 Å (30 nm) pore size packing material. The larger pore size is recommended for the analysis of mid-sized and large-sized molecules with molecular weights above 5000 to enable retention and avoid size-exclusion effects. This analysis of a mixed sample of four different peptides and proteins demonstrated good peak shapes and favorable separation of insulin and ribonuclease A due to the pore size that was large enough to allow proper diffusion of these large molecular weight compounds.  

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Shim-pack Scepter C4-300 is wide-pore organic silica hybrid reversed phase columns recommended for the separation of proteins such as monoclonal antibodies and mid-sized molecules such as oligonucleic acids and peptides that may not be retained on 100-120Å pore size columns due to size-exclusion effects. The organic silica hybrid base material is highly stable even at high temperatures under acidic and basic mobile phase conditions. Pore sizes are optimized for large molecular weight compounds and dispersed uniformly in the column, resulting in symmetrical peak shapes and increased resolution of antibodies and nucleic acids compared to a smaller pore size column. Shim-pack Scepter C4-300 is also effective for high-sensitivity LC/MS analysis, producing good peak shapes even under weak ionpairing conditions using a formic acid mobile phase Peptide Analysis Example Shim-pack Scepter C18-300 and C4-300 made with 300 Å (30 nm) pore size packing material. The larger pore size is recommended for the analysis of mid-sized and large-sized molecules with molecular weights above 5000 to enable retention and avoid size-exclusion effects. This analysis of a mixed sample of four different peptides and proteins demonstrated good peak shapes and favorable separation of insulin and ribonuclease A due to the pore size that was large enough to allow proper diffusion of these large molecular weight compounds.  

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Shim-pack Scepter C4-300 is wide-pore organic silica hybrid reversed phase columns recommended for the separation of proteins such as monoclonal antibodies and mid-sized molecules such as oligonucleic acids and peptides that may not be retained on 100-120Å pore size columns due to size-exclusion effects. The organic silica hybrid base material is highly stable even at high temperatures under acidic and basic mobile phase conditions. Pore sizes are optimized for large molecular weight compounds and dispersed uniformly in the column, resulting in symmetrical peak shapes and increased resolution of antibodies and nucleic acids compared to a smaller pore size column. Shim-pack Scepter C4-300 is also effective for high-sensitivity LC/MS analysis, producing good peak shapes even under weak ionpairing conditions using a formic acid mobile phase Peptide Analysis Example Shim-pack Scepter C18-300 and C4-300 made with 300 Å (30 nm) pore size packing material. The larger pore size is recommended for the analysis of mid-sized and large-sized molecules with molecular weights above 5000 to enable retention and avoid size-exclusion effects. This analysis of a mixed sample of four different peptides and proteins demonstrated good peak shapes and favorable separation of insulin and ribonuclease A due to the pore size that was large enough to allow proper diffusion of these large molecular weight compounds.  

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Shim-pack Scepter C4-300 is wide-pore organic silica hybrid reversed phase columns recommended for the separation of proteins such as monoclonal antibodies and mid-sized molecules such as oligonucleic acids and peptides that may not be retained on 100-120Å pore size columns due to size-exclusion effects. The organic silica hybrid base material is highly stable even at high temperatures under acidic and basic mobile phase conditions. Pore sizes are optimized for large molecular weight compounds and dispersed uniformly in the column, resulting in symmetrical peak shapes and increased resolution of antibodies and nucleic acids compared to a smaller pore size column. Shim-pack Scepter C4-300 is also effective for high-sensitivity LC/MS analysis, producing good peak shapes even under weak ionpairing conditions using a formic acid mobile phase Peptide Analysis Example Shim-pack Scepter C18-300 and C4-300 made with 300 Å (30 nm) pore size packing material. The larger pore size is recommended for the analysis of mid-sized and large-sized molecules with molecular weights above 5000 to enable retention and avoid size-exclusion effects. This analysis of a mixed sample of four different peptides and proteins demonstrated good peak shapes and favorable separation of insulin and ribonuclease A due to the pore size that was large enough to allow proper diffusion of these large molecular weight compounds.  

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Shim-pack Scepter C4-300 is wide-pore organic silica hybrid reversed phase columns recommended for the separation of proteins such as monoclonal antibodies and mid-sized molecules such as oligonucleic acids and peptides that may not be retained on 100-120Å pore size columns due to size-exclusion effects. The organic silica hybrid base material is highly stable even at high temperatures under acidic and basic mobile phase conditions. Pore sizes are optimized for large molecular weight compounds and dispersed uniformly in the column, resulting in symmetrical peak shapes and increased resolution of antibodies and nucleic acids compared to a smaller pore size column. Shim-pack Scepter C4-300 is also effective for high-sensitivity LC/MS analysis, producing good peak shapes even under weak ionpairing conditions using a formic acid mobile phase Peptide Analysis Example Shim-pack Scepter C18-300 and C4-300 made with 300 Å (30 nm) pore size packing material. The larger pore size is recommended for the analysis of mid-sized and large-sized molecules with molecular weights above 5000 to enable retention and avoid size-exclusion effects. This analysis of a mixed sample of four different peptides and proteins demonstrated good peak shapes and favorable separation of insulin and ribonuclease A due to the pore size that was large enough to allow proper diffusion of these large molecular weight compounds.  

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Shim-pack Scepter C4-300 is wide-pore organic silica hybrid reversed phase columns recommended for the separation of proteins such as monoclonal antibodies and mid-sized molecules such as oligonucleic acids and peptides that may not be retained on 100-120Å pore size columns due to size-exclusion effects. The organic silica hybrid base material is highly stable even at high temperatures under acidic and basic mobile phase conditions. Pore sizes are optimized for large molecular weight compounds and dispersed uniformly in the column, resulting in symmetrical peak shapes and increased resolution of antibodies and nucleic acids compared to a smaller pore size column. Shim-pack Scepter C4-300 is also effective for high-sensitivity LC/MS analysis, producing good peak shapes even under weak ionpairing conditions using a formic acid mobile phase Peptide Analysis Example Shim-pack Scepter C18-300 and C4-300 made with 300 Å (30 nm) pore size packing material. The larger pore size is recommended for the analysis of mid-sized and large-sized molecules with molecular weights above 5000 to enable retention and avoid size-exclusion effects. This analysis of a mixed sample of four different peptides and proteins demonstrated good peak shapes and favorable separation of insulin and ribonuclease A due to the pore size that was large enough to allow proper diffusion of these large molecular weight compounds.  

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