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Shim-pack Scepter Claris PFPP-120, 3um, 4.6x50

Part Number: 227-31214-07

Column Name: Scepter Claris PFPP-120

Particle size (µm): 3

ID (mm): 4.6

Length (mm): 50

USP: L43

Functional Group: PFPP

Mode: Reversed Phase

Type: Analytical column (Bioinert)

Pore size (nm): 12

End Capping: No

Carbon Load (%): 15

PH: 1 to 8

Pressure Tolerance (MPa): 45

Pack size: 1

Lead Time: 4 - 6 weeks

Shim-pack Scepter Claris features a column body with a newly-developed bioinert coating packed with Scepter series stationary phases.

  • Bioinert coating is applied to the column body and stainless steel frit
  • Ideal for analysis of metal-coordinating and hydrophobically adsorbing compounds such as nucleic acids, proteins, and lipids
  • Outstanding pH and lifetime stability due to Scepter organic silica hybrid packing

 

Superior Sensitivity and Separation Performance in Nucleic Acid Analysis

Shim-pack Scepter Claris C18-120 with the bioinert coating and Scepter C18-120 with traditional stainless steel hardware were compared in this example of an analysis of a synthetic oligonucleotide. Results from Claris were highly sensitive and reproducible from the rst injection, with no loss of sample signal. Scepter C18-120 in a stainless steel column body produced low-sensitivity results and showed adsorption from the first sample injection.

Outstanding Performance in Oligonucleic Acid Analysis

Shim-pack Scepter Claris C18-120 (bioinert coating) and Scepter C18-20 (stainless steel body) were compared in this analysis of Oligodeoxythymidylic acid [d(T)20]. Results from Scepter C18-120 show low peak intensity, suggesting adsorption on metal surfaces. In contrast, Scepter Claris C18-120 produced a sharper and high-intensity peak.

Improved Sensitivity and Separation of Phospholipids

Shim-pack Scepter Claris C18-120 minimizes metal-mediated adsorption and hydrophobic absorption on the column surfaces, resulting in high-resolution separations. In this example of structurally complex phosphatidylcholine isomers, Scepter Claris C18-120 achieved increased baseline resolution between isomers with higher sensitivity.

 

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